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opal tsa dig reagent  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences opal tsa dig reagent

    Opal Tsa Dig Reagent, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opal+reagents/TSA+Plus+DIG/pmc13675480-525-12-15
    Average 94 stars, based on 28 article reviews
    opal tsa dig reagent - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa"

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    Journal: iScience

    doi: 10.1016/j.isci.2026.117730


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    Related Articles

    Amplification:

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype.
    Article Snippet: Objective: High dose acetaminophen (AAP) has demonstrated promising results in early phase clinical trials for treatment of advanced malignancies.. When administered concurrently with the CYP2E1 inhibitor fomepizole, AAP can be dose-escalated 100-fold relative to standard dosing without liver toxicity and without compromising anti-tumor activity.. Methods and analysis: The current study used a 23-plex flow cytometry panel to study AAP-induced changes in the tumor immune microenvironment of syngeneic mouse breast tumors.

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype
    Article Snippet: .. The Opal reagents (Akoya Biosciences) were diluted in 1X+ automation amplification diluent, except Opal 780, as specified in Supplementary Table 3 and incubated for 10 min. Opal 780 was diluted in Blocking/Ab Diluent (Akoya Biosciences) and incubated for 10 min. .. The Opal incubations for 520, 570, 690, and 780 were repeated a second time for 10 min. All slides were cover-slipped with Prolong Diamond Antifade mounting media (ThermoFisher Scientific) and digitally scanned using Pheno-Imager HT (Akoya Biosciences) at a magnification of 20X.

    Article Title: Metabolic targeting of cancer associated fibroblasts overcomes T-cell exclusion and chemoresistance in soft-tissue sarcomas.
    Article Snippet: Finally, the membranes were incubated with ECL substrate (Pierce) for 1min and exposed for signal detection with the iBright Imager (Thermo Fisher). .. Tissue processing and multiplex immunohistochemistry Immunofluorescence staining utilized tyramide signal amplification staining performed using OPAL Reagents (Akoya Biosciences, Inc, Marlborough, MA). ..

    Incubation:

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype.
    Article Snippet: Objective: High dose acetaminophen (AAP) has demonstrated promising results in early phase clinical trials for treatment of advanced malignancies.. When administered concurrently with the CYP2E1 inhibitor fomepizole, AAP can be dose-escalated 100-fold relative to standard dosing without liver toxicity and without compromising anti-tumor activity.. Methods and analysis: The current study used a 23-plex flow cytometry panel to study AAP-induced changes in the tumor immune microenvironment of syngeneic mouse breast tumors.

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype
    Article Snippet: .. The Opal reagents (Akoya Biosciences) were diluted in 1X+ automation amplification diluent, except Opal 780, as specified in Supplementary Table 3 and incubated for 10 min. Opal 780 was diluted in Blocking/Ab Diluent (Akoya Biosciences) and incubated for 10 min. .. The Opal incubations for 520, 570, 690, and 780 were repeated a second time for 10 min. All slides were cover-slipped with Prolong Diamond Antifade mounting media (ThermoFisher Scientific) and digitally scanned using Pheno-Imager HT (Akoya Biosciences) at a magnification of 20X.

    Blocking Assay:

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype.
    Article Snippet: Objective: High dose acetaminophen (AAP) has demonstrated promising results in early phase clinical trials for treatment of advanced malignancies.. When administered concurrently with the CYP2E1 inhibitor fomepizole, AAP can be dose-escalated 100-fold relative to standard dosing without liver toxicity and without compromising anti-tumor activity.. Methods and analysis: The current study used a 23-plex flow cytometry panel to study AAP-induced changes in the tumor immune microenvironment of syngeneic mouse breast tumors.

    Article Title: High dose acetaminophen re-polarizes CD11b+ cells in the tumor microenvironment towards an activated macrophage phenotype
    Article Snippet: .. The Opal reagents (Akoya Biosciences) were diluted in 1X+ automation amplification diluent, except Opal 780, as specified in Supplementary Table 3 and incubated for 10 min. Opal 780 was diluted in Blocking/Ab Diluent (Akoya Biosciences) and incubated for 10 min. .. The Opal incubations for 520, 570, 690, and 780 were repeated a second time for 10 min. All slides were cover-slipped with Prolong Diamond Antifade mounting media (ThermoFisher Scientific) and digitally scanned using Pheno-Imager HT (Akoya Biosciences) at a magnification of 20X.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Favorable histo-molecular remodeling of pancreatic ductal adenocarcinoma after Total Neoadjuvant Therapy including Stereotactic Body Radiotherapy
    Article Snippet: Quantification of the different stainings was performed with the Visiopharm © software. .. FFPE tissue sections (4μm) were processed manually for mIHC using Opal reagents (Akoya Biosciences) for illustration purposes in four representative samples (2 in the No-NAT and TNT groups). ..

    Multiplex Assay:

    Article Title: Metabolic targeting of cancer associated fibroblasts overcomes T-cell exclusion and chemoresistance in soft-tissue sarcomas.
    Article Snippet: Finally, the membranes were incubated with ECL substrate (Pierce) for 1min and exposed for signal detection with the iBright Imager (Thermo Fisher). .. Tissue processing and multiplex immunohistochemistry Immunofluorescence staining utilized tyramide signal amplification staining performed using OPAL Reagents (Akoya Biosciences, Inc, Marlborough, MA). ..

    Immunohistochemistry:

    Article Title: Metabolic targeting of cancer associated fibroblasts overcomes T-cell exclusion and chemoresistance in soft-tissue sarcomas.
    Article Snippet: Finally, the membranes were incubated with ECL substrate (Pierce) for 1min and exposed for signal detection with the iBright Imager (Thermo Fisher). .. Tissue processing and multiplex immunohistochemistry Immunofluorescence staining utilized tyramide signal amplification staining performed using OPAL Reagents (Akoya Biosciences, Inc, Marlborough, MA). ..

    Immunofluorescence:

    Article Title: Metabolic targeting of cancer associated fibroblasts overcomes T-cell exclusion and chemoresistance in soft-tissue sarcomas.
    Article Snippet: Finally, the membranes were incubated with ECL substrate (Pierce) for 1min and exposed for signal detection with the iBright Imager (Thermo Fisher). .. Tissue processing and multiplex immunohistochemistry Immunofluorescence staining utilized tyramide signal amplification staining performed using OPAL Reagents (Akoya Biosciences, Inc, Marlborough, MA). ..

    Staining:

    Article Title: Metabolic targeting of cancer associated fibroblasts overcomes T-cell exclusion and chemoresistance in soft-tissue sarcomas.
    Article Snippet: Finally, the membranes were incubated with ECL substrate (Pierce) for 1min and exposed for signal detection with the iBright Imager (Thermo Fisher). .. Tissue processing and multiplex immunohistochemistry Immunofluorescence staining utilized tyramide signal amplification staining performed using OPAL Reagents (Akoya Biosciences, Inc, Marlborough, MA). ..



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Signal development of the HRP-conjugated antibody was achieved by stepwise incubation with Opal TSA-DIG Reagent (Akoya Biosciences) diluted 1:250 in RNAscope Multiplex TSA Buffer for 10 minutes at RT, RNAscope Multiplex FL v2 HRP Blocker for 25 min at 40 °C in the HybEZ oven, and Opal 780 Reagent (Akoya Biosciences) diluted 1:62,5 in 1x Antibody Diluent/Block (Akoya Biosciences) for 10 min at RT.

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy